Sliding strip microfluidic device enables ELISA on paper. This article describes a 3. D microfluidic paper based analytical device that can be used to conduct an enzyme linked immunosorbent assay ELISA. The device comprises two parts a sliding strip which contains the active sensing area and a structure surrounding the sliding strip which holds stored reagentsbuffers, antibodies, and enzymatic substrateand distributes fluid. Running an ELISA involves adding sample e. We demonstrate that this device can be used to detect C reactive protein CRPa biomarker for neonatal sepsis, pelvic inflammatory disease, and inflammatory bowel diseasesat a concentration range of 11. Mark_Sarvary/publication/273457358/figure/download/fig5/AS:294798566150148@1447296863490/Figure-7-Serial-Dilution-of-an-initial-sample-or-culture-to-obtain-solutions-that-are.png' alt='Example Four Fold Serial Dilution' title='Example Four Fold Serial Dilution' />The following information is an updated version of a method for using ImageJ to analyze western blots from a nowdeprecated older page. If youre looking for a more. Free Burn Fat Feed Muscle Book. D microfluidic paperbased analytical device performs ELISA with colorimetric results. Two components enable separation of reagents in the device a sliding. Steps to validate flow cytometry results and improve reproducibility. For scientific results to be valuable, they must be reproducible. There is significant concern. L in 1. 00. 0 fold diluted blood 11. L in undiluted blood. The accuracy of the device as characterized by the area under the receiver operator characteristics curve is 8. L for neonatal sepsis and pelvic inflammatory disease and 3. L for inflammatory bowel diseases CRP in 1. In resource limited settings, the device can be used as a part of a kit containing the device, a fixed volume capillary, a pre filled tube, a syringe, and a dropper this kit would cost 0. This kit has the technical characteristics to be employed as a pre screening tool, when combined with other data such as patient history and clinical signs.